Name of supervisor： Takeharu Kaneda, Ph. D. (Veterinary Medicine)
Position: Professor
researchmap
KAKEN researcher number: 10350175
ORCID ID：0000-0002-4571-2701
Main research theme: Pharmacological analysis of smooth muscle function ex vivo
Research keywords: smooth muscle contraction・drug repositioning・vascular endothelial cell
Lab location: Laboratory of Veterinary Pharmacology, 4th floor, Building D
E-mail：t-kaneda (@mark)nvlu.ac.jp
Research
Smooth muscle cells are distributed in various organs, including the circulatory organs such as blood vessels, the gastrointestinal tract such as the stomach and intestines, and the genitourinary organs such as the uterus, seminal vessels, prostate, urethra, and bladder. Smooth muscle cells also play a very important role in the physiological functions of internal organs and sensory organs except the heart, such as gastrointestinal motility, blood pressure regulation, urinary and reproductive system regulation, and so on. Smooth muscle cells, even very small spindle-shaped cells, do not differ histologically from organ to organ, but they also cooperate with other cells, and aggregates of multiple cells have different organ functions and drug sensitivity.
We have appeared that the relationship between the vasorelaxant effects of some polyphenols and novel diabetes drugs and endothelial cell function, and have found that these drugs release nitric oxide (NO) synthesis, which is known to prevent heart failure and atherosclerosis, using ex vivo techniques to clarify the mechanism via activating NO synthase (eNOS). The mechanism of eNOS activation has been the subject of many theories and has not been fully elucidated. We believe that examining the activation mechanism of eNOS with multiple drugs may clarify the standard of the activation mechanism of eNOS.
We are also conducting functional analysis of the relationship between smooth muscle contraction and phosphodiesterase isozymes in various organs, and are conducting research that leading to the development of new drugs.
Guidance policy for graduate students
So far, I have supervised one graduate student in the Doctoral Program in Veterinary Medicine and one graduate student in the Master’s Program in Veterinary Health Nursing. Our teaching policy is to help students acquire the ability to process and think for themselves from a variety of information.
The research in this laboratory is characterized by starting with the phenotype of chemical agents-induced smooth muscle contractile responses. The ultimate goal is finding out the mechanism of chemical agents causing smooth muscles contraction or relaxation. In order to clarify that, we have tried to proceed while engaging in dialogue with students, starting from the point of researching information such as “what experimental methods are available” and “what kind of inhibitors should be used. Even when students are not presenting their own research, I try to give them opportunities to attend academic conferences, see presentations by other researchers, and learn about the latest research and the thinking of other researchers, so that they can gain as broad a knowledge and perspective as possible.
Although basic research is more modest than clinical research and does not produce results that are immediately useful to the world, I believe that it is our responsibility as a graduate supervisor to provide guidance to my students so that they can be proud of how their research has contributed to the field of life science.
Publications
1. Kanda H, Kaneda T, et al. 2016. (First graduate student author, Ph.D. in Veterinary Medicine, 2016)
Aerobic metabolism on muscle contraction in porcine iris sphincter.
J Vet Med Sci. 2016;78(11):1673-1676.
doi: 10.1292/jvms.16-0175
Analysis of pig iris sphincter contraction in relation to aerobic metabolism.
2. Kanda H, Kaneda T, et al. 2017.(First graduate student author, PhD in Veterinary Medicine, 2016)
Phloridzin inhibits high K+-induced contraction via the inhibition of sodium: Glucose cotransporter 1 in rat ileum.
J Vet Med Sci. 2017;79(3):593-601.
doi: 10.1292/jvms.16-0560.
Analysis of the relationship between ileal smooth muscle contraction and SGLT1-mediated glucose dynamics.
3. Kaneda H, Otomo R, Sasaki N, Omi T, Sato T, Kaneda T. 2019
(First author, graduate student, Master of Science in Veterinary Health Nursing, 2018)
Endothelium-independent vasodilator effects of nobiletin in rat aorta.
J Pharmacol Sci. 2019 140(1):48-53. doi: 10.1016/j.jphs.2019.04.004.
Guanylate cyclase activation and ATP-sensitive K-channel opening are associated with the mechanism of nobiletin-induced vasorelaxation in rats.
