Research
The reproductive physiology of dogs differs from that of other mammals in many unique ways. A comprehensive understanding of these traits is essential for successful breeding. Our research endeavors to apply the findings from these studies clinically.
Of particular interest is our investigation into frozen dog semen, an area where research has lagged behind that of other mammals. Canine sperm exhibits lower freezing tolerance and is more susceptible to damage from low temperatures compared to other animals. Our research on frozen dog semen has revealed that a high conception rate can be achieved through surgical intrauterine insemination using frozen-thawed semen. However, the surgical method causes stress on the animal, necessitating the development of a nonsurgical transcervical intrauterine insemination. However, the current lifespan of frozen dog semen post-thawing is brief, and in transcervical intrauterine insemination, sperm may not be able to reach the oviduct, resulting in slightly lower pregnancy rates compared to surgical methods. Moreover, this nonsurgical method requires expensive equipment and technical skills.
Considering dogs’ monoestrous nature and long estrous cycles, infertility can represent a considerable loss in their fertile age. Therefore, ensuring pregnancy through artificial insemination with frozen semen becomes. We believe it is imperative to enhance the qualities of frozen dog semen after thawing, aiming for improves sperm motility and extended lifespan. By doing so, we aspire not to only increase the conception rate through transcervical intrauterine insemination, previously challenging, a viable option. We believe that the progress of these studies could address the global need for assistance dogs.
Furthermore, we engage in joint research with zoos and breeding facilities, focusing on the artificial breeding of rare wild animals such Felids and Canids. We apply our expertise in assisted reproductive technologies developed for dogs and cats.
Our laboratory includes Associate Professor Masanori Kobayashi and lecturer Masato Kobayashi. Associate Professor Masanori Kobayashi’s research focuses on establishing the mechanism of onset of prostate cancer in dogs, early diagnosis methods, new treatment methods, treatment of reproductive dysfunction, and elucidation of testicular cancer pathogenesis. Lecturer Masato Kobayashi primarily investigates fetal immune tolerance involved in maintaining pregnancy in dogs and its clinical applications.