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Hiroshi AOKI, DVM, PhD
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| Position |
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Professor |
| KAKEN Researcher Number |
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10440067 |
| Research Keywords |
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Animal Viral Infections, Pestivirus Infections (Virology, Molecular virology, Epidemiology, Diagnostic Methods, Vaccine, and Infection control), Animal Parvovirus Infections, Animal Circovirus Infections. |
| Lab. Location |
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Department of Basic Science
4th Floor, Building E |
| E-mail |
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aokihir(@mark)nvlu.ac.jp |
Research
Our research focuses on animal viral infections, which are familiar or routine outbreaks and problems in Japan, with the aim of preventing, controlling, or eliminating them from a multifaceted perspective and with the use of technology.
In particular, we focus on basic and practical research on pestivirus infections caused by bovine viral diarrhea virus (BVDV), atypical porcine pestivirus (APPeV), and border disease virus (BDV) of the genus Pestivirus, and also conduct surveys and research that contribute to control measures for classical swine fever virus (CSFV). Our BVDV and APPeV research is one of the best in Japan. Other non-enveloped small spherical viruses (such as canine, feline, and swine parvoviruses and porcine circovirus) are also investigated and researched, and we are working on topics related to diagnosis and prevention that are essential in clinical practice.
To overcome these infectious diseases, we are actively introducing various technologies such as tissue culture, molecular virology, molecular biology, pathology, genetic recombination, seroepidemiology, and epidemiology (statistics and analysis) to accumulate results supported by scientific evidence.
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Research on pathogenicity, innate immune regulation, quasispecies and interference phenomena of pestiviruses (BVDV and APPeV) |
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Research on host specificity of pestiviruses (BVDV, BDV and APPeV) and the mechanism of acquisition of infection to heterologous animals |
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Comprehensive research on newly discovered APPeV |
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Basic development of new vaccines and diagnostic agents (pestiviruses and circovirus) |
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Surveillance and research on infection control (evaluation of the effectiveness of virus inactivation and disinfection, investigation of transmission routes, etc.) |
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Outbreak surveillance (surveys using a combination of biological and molecular epidemiological techniques) |
Guidance Policy for Graduate Students
Our basic policy is to contribute to society and veterinary medicine as veterinary paraprofessionals (VPP) including veterinary nurses for companion animals. On top of that, students will be trained to be capable of practicing animal hygiene or animal infection control as a subspecialty, and to be able to conduct surveys and research in a professional manner.
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Master’s Program: |
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Research guidance with an emphasis on acquiring the basic skills necessary for research |
Doctoral Course: |
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Research guidance with an emphasis on promoting independent and practical research and career development. |
Note
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Since we are constantly conducting research on pathogenic viruses and genetic recombination experiments, applicants should already have the following basic knowledge and skills are desirable. |
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Tissue culture techniques (e.g., maintenance of primary cells and cell lines) |
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Microbiology standard techniques required for Biosafety Level 2 |
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Experimental techniques for genetic recombination experiments at Level P2 of the Proliferation Prevention Measures |
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We are scientifically pursuing the life phenomenon of “viral infections”. To promote measures against pestivirus infections, our activities range from basic research to applied research such as the development of diagnostic and preventive methods. We welcome those who are willing to work seriously with us to scientifically elucidate what viral infections are in animals and to protect animals from viral infections! |
Publications
| 1. |
Mai Shiokawa, Yui Morita, Makoto Nagai, Makoto Haritani, Hiroshi Aoki. (2023).
Isolation and artificial production of atypical porcine pestivirus, using the swine-kidney-derived cell line SK-L.
Arch Virol, 168(12), 294.
DOI: 10.1007/s00705-023-05919-y |
| 2. |
Moe Shioda, Mai Shiokawa, Hiroshi Aoki. (2023).
(First author: Undergraduate students)
Establishment of guinea pig kidney cell lines with potential application in the production of a classical swine fever live GPE− vaccine.
J Vet Med Sci, 85(3), 308-317.
DOI: 10.1292/jvms.22-0385 |
| 3. |
Mai Shiokawa, Tsutomu Omatsu, Hiroshi Aoki, et al. (2019).
END-phenomenon negative bovine viral diarrhea virus that induces the host’s innate immune response supports propagation of BVDVs with different immunological properties.
Virology, 583, 97-110.
DOI: 10.1016/j.virol.2019.09.016 |
| 4. |
Shuhei Hosono, Mai Shiokawa, Tsubasa Kobayashi, Akio Fukusho, Hiroshi Aoki. (2019).
(First author: Graduate students)
Porcine circovirus type 2 induces a strong cytopathic effect in the serum-free culture cell line CPK-NS cells.
J Virol Methods, 273, 113706.
DOI: 10.1016/j.jviromet.2019.113706 |
| 5. |
Hiroshi Aoki, Fujiko Sunaga, Hideharu Ochiai et al. (2019).
Phylogenetic analyses of novel posaviruses detected in feces of Japanese pigs with posaviruses and posa-like viruses of vertebrates and invertebrates.
Arch Virol, 164(8), 2147-2151.
DOI: 10.1007/s00705-019-04289-8 |
| 6. |
Kaoru Nishine, Hiroshi Aoki, Yoshihiro Sakoda, Akio Fukusho. (2014).
(First author: Graduate students)
Field Distribution of Exaltation of Newcastle disease virus phenomenon-negative bovine viral diarrhea virus.
J Vet Med Sci, 76(12), 1635-1639.
DOI: 10.1292/jvms.14-0220 |
| 7. |
Hiroshi Aoki, Kiyoyasu Ishikawa, Hideto Sekiguchi, Shoko Suzuki, Akio Fukusho. (2003).
Pathogenicity and kinetics of virus propagation in swine infected with the cytopathogenic classical swine fever virus containing defective interfering particles.
Arch Virol, 148(2), 297-310.
DOI: 10.1007/s00705-002-0907-2 |
| 8. |
Hiroshi Aoki, Kiyoyasu Ishikawa, Yoshihiro Sakoda, Hideto Sekiguchi, Michi Kodama, Shoko Suzuki, Akio Fukusho. (2001).
Characterization of classical swine fever virus associated with defective interfering particles containing a cytopathogenic subgenomic RNA isolated from wild boar.
J Vet Med Sci, 63(7), 751-758.
DOI: 10.1292/jvms.63.751 |
Detailed Information (Researchmap)
