Name of supervisor：Atsushi Tohei
Position：Professor
researchmap
KAKEN researcher number: 70307685
Main research theme：
１）Physiological responses to stress in rodents.
２）The 3Rs in animal research (Replacement, Reduction and Refinement)
Research keywords： Stress, The 3Rs, anesthesia
E-mail：tohei(@mark)nvlu.ac.jp
Research
One of the most crucial aspects of laboratory animal science is understanding the fundamentals of the 3Rs principles (Replacement, Reduction, and Refinement) of animal testing. In the 3Rs, refinement describes techniques that reduce pain or stress, and it is difficult to set the strategies aimed at achieving refinement without understanding the pain or stress responses in laboratory animals. As a result, I’m interested in studying the physiological reactions of animals, particularly mice, to stress. Using the proper anesthesia and analgesia to reduce pain are examples of refinement; anesthetic techniques are also within the scope of my research interests.
Guidance policy for graduate students
The slogans of my laboratory are listed below.
1. Consider yourself a mouse thinking on the basis of experimental data.
2. Obtain information that could help laboratory animal scientists achieve the 3Rs.
Experimental methods include behavioral analysis, HPLC with ECD detection for monoamine (NE, DA, and 5-HT), EIA for the detection of the stress hormone (corticosterone), and activity and temperature analysis using monitoring devices such as nano tag® in mice.
Publications
1.Recommended doses of medetomidine-midazolam-butorphanol with atipamezole for preventing hypothermia in mice. Mizuho Tashiro, Atsushi Tohei., The Journal of veterinary medical science 84(3) 445-453, 2022
2.Duration of thermal support for preventing hypothermia induced by anesthesia with medetomidine-midazolam-butorphanol in mice. Mizuho Tashiro, Yuki Hosokawa, Hiromi Amao, Atsushi Tohei, The Journal of veterinary medical science 82(12):1757-1762, 2020
3.Evaluation of response to restraint stress by salivary corticosterone levels in adult male mice. Masakatsu Nohara, Atsushi Tohei, Takumi Sato, Hiromi Amao, The Journal of veterinary medical science 78(5) 775-780, 2016
4.Studies on the functional relationship between thyroid, adrenal and gonadal hormones. Atsushi Tohei., The Journal of Reproduction and Development 50(1) 9-20, 2004
5.Hypothyroidism increases prolactin secretion and decreases the intromission threshold for induction of pseudopregnancy in adult female rats. A Tohei, K Taya, G Watanabe, JL Voogt., PHYSIOLOGY & BEHAVIOR 69(4-5) 391-397 2000
